Om Prakash Singh, PhD
Hi, I am a scientist who retired recently from the National Institute of Malaria Research., New Delhi
Recent Publications (2022-23)
Singh OP*, Kaur T, Sharma G, Kona MP, Mishra S, Mallick PK.
There are increasing reports of the invasion of the Asian malaria vector Anopheles stephensi in several parts of the world. We report the development of molecular methods (Real-Time PCR, size-diagnostic PCR and DNA sequencing methods) for detecting a single An. stephensi in large pools of mosquitoes of mixed species which will help in the early detection of invasion of this species in a new geographical area.
Yadav K, Rana VS, Kaushik A, Saurav GK, Rawat N, Kumar A, Sunil S, Singh OP, Rajagopal R
In this study, we focused on the interaction between dengue virus (DENV) virions and candidate proteins of Ae. aegypti. To identify such candidate proteins, phage-display library screening was performed against domain III of the envelope protein (EDIII) serotype DENV-2. A peptide sequence for this screening showing similarity to mucin protein was found to interact with EDIII of DENV-2. Mucin protein was cloned, expressed and subsequently purified for in vitro protein-protein interaction studies. Mucin protein was localized in the midgut of the Ae. aegypti by using confocal microscopy and further, blocking of mucin protein in Ae. aegypti with anti-mucin antibodies resulted in 12.5% reduction in the DENV replication.
Sindhania A, Lohani HP, Kona MP, Kaur T, Kaushal BR, Singh OP*
PLoS ONE. 2023; 18(2): e0280289 doi: 10.1371/journal.pone.0280289
This study reports significant differences in insecticide susceptibility between two molecular forms of An. subpictus complex (A and B) in sympatric populations. The L1014F-kdr mutation was observed in Form A only, which was associated with DDT, permethrin and deltamethrin resistance.
Dykes CL, Sharma G, Behera AK, Kapoor N, Paine MJI, Donnelly MJ, Singh OP*.
Scientific Reports.. 2022; 12: 17872. doi: 10.1038/s41598-022-21522-8
We identified tandemly-arrayed duplication of a 3.62 kb segment of GST epsilon gene clusters comprised of five genes: a partial GSTe1, GSTe2, GSTe2-pseudogene, GSTe4 and partial GSTe5, interconnected by a conserved 2.42 kb DNA insert segment major part of which is homologous to a genomic region located on a different chromosome. The tandemly duplicated array contained a total of two GSTe2 and three GSTe4 functional paralog genes. The increased gene dose in mosquitoes as a result of the GSTe gene duplication may be an adaptive process to increase levels of detoxifying enzymes to counter insecticide pressure.
Singh OP*, Mishra S, Sindhania A, Kaur T, Srihari U, Das MK, Kapoor N, Sharma G, Gupta B.
PLoS ONE.2022; 17 (7): e0270760. doi: 10.1371/journal.pone.0270760
Recently, the first intron of the odorant-binding protein-1 (AsteObp1) has been introduced as a molecular marker for the identification of biological forms of An. stephensi, and based on this marker, the presence of three putative sibling species (designated as species A, B and C) was proposed. In this study, we showed high heterozygosity for this marker and a low inbreeding coefficient in field and laboratory populations indicating that this marker is not suitable for the delimitation of putative sibling species, at least in Indian populations
Kumari V, Prasad KM, Kalia I, Sindhu G, Dixit R, Rawat DS, Singh OP, Singh AP, Pandey KC*
Our findings indicate that the deletion of MCA-2 hampers the Plasmodium development during erythrocytic and exo-erythrocytic stages, and its inhibition by C-532 and C-533 critically affects malaria transmission biology.
Mallick PK, Sindhania A, Gupta T, Singh DP, Saini S, Singh OP*.
Medical and Veterinary Entomology; 2022, July 15; doi: 10.1111/mve.12596
We reported the presence of four kdr mutations: M827I, T929I, L932F and L1014F in Indian head louse. The presence of a classical kdr mutation L1014F, the most widely reported mutation across insect-taxa associated with the kdr-trait, is being reported for the first time in the head louse.
Carlton JM*, Eapen A, Kessler A, Anvikar AR, Hoffmann A, Singh OP, Sullivan SA, Albert S, Sahu PK, Mohanty S, Wassmer SC
American Journal of Tropical Medicine & Hygiene. 2022; 107:97–106 doi: 10.4269/ajtmh.21-1333
The Center for the Study of Complex Malaria in India (CSCMi) is one of 10 International Centers of Excellence in Malaria Research funded by the National Institutes of Health since 2010. The Center combines innovative research with capacity building and technology transfer to undertake studies with clinical and translational impact that will move malaria control in India toward the ultimate goal of malaria elimination/eradication. This article provides a snapshot of some of the basic and applied research the CSCMi has undertaken over the past 12 years and indicate the further research and/or clinical and translational impact these studies have had.
Lather M, Mallick PK, Sharma D, Kale S, Dang AS, Adak T, Singh, OP
Medical and Veterinary Entomology; 2022, 36:194-202. doi: 10.1111/mve.12566
This study inferred a genetic structure among six geographically diverse populations of species T using a panel of microsatellite markers. Analyses indicated a significant but low genetic differentiation among the majority of the studied populations. A significant correlation was observed between genetic and geographic distances, exhibiting stepwise migration patterns among populations.
Vandana V, Kona MP, Kumar J, Singh OP, Pandey KC
Experimental Parasitology. 2022; 243:108407. doi: 10.1016/j.exppara.2022.108407
This article summarizes an overview of the previously studied microbial symbionts for their role in Plasmodium transmission along with a list of new or unexplored symbionts in the disease-transmitting mosquito vectors. The summarized information could be utilized to explore such microbial symbionts for their role in Plasmodium-transmission biology in-depth and implementation in the malaria control interventions globally.
Surendran SN, Kesavan L, Jayadas TPP, Sivabalakrishnan K, Tharsan A, Liyanagedara N, Eswaramohan T, Raveendran S, Singh OP, Ramasamy R
Medical & Veterinary Entomology. 2022; 34:496-502; doi: 10.1111/mve.12595
Verma S, Chakraborti S, Singh OP, Pande V, Dixit R, Pandey AV, Pandey KC
FrontIers in Endocrinology. 2022; 13:981090.; doi: 10.3389/fendo.2022.981090
Baharia RK, Kaur J, Sindhania A, Sharma G, Srivastava HC, Pant CS, Rahi M, Raghavendra K, Singh OP
Journal of Arthropod Borne Diseases. 2022; 16(2): 108–123. doi: 10.18502/jad.v16i2.11802
Sumitha MK, Kalimuthu M, Kumar MS, Rajaiah P, Pradeep NK, Sunish IP, Balaji T, Devojit KS, Goyal D, Suman DS, Srivastava H, Bhowmik IP, Vaishnav K, Singh OP, Patil P, Tyagi S, Mohanty S, Barik TK, Sreehari U, Kumar A, Gupta B
PLoS Neglected Tropical Diseases. 2023; 17(7):e0011486:. doi:10.1371/journal.pntd.0011486
Atul, Choudhary P, Gupta S, Shoaib R, Pasupureddy R, Gotal B, Kumar B, Singh OP, Dixit R, Singh S, Akhtar, M, Kapoor N, Pande V, Chakraborti S, Vashishth K, Pandey KC
Journal of Global Antimicrobial Resistance . 2023; Aug 24, S2213-7165(23)00137-6. doi: 10.1016/j.jgar.2023.08.012,
Kale S, Uplekar SM, Bandyopadhyay N, Rao PN, Ali SZ, Sharma SK, Tandel N, Patel A, Singh R, Dank A, Ravishankaran S, Lakshmi Priya GS, Asokan A, Eapen A, Singh OP, Carlton JM✻, Mallick PK
Singh OP, Kaur T
Scientific Reorts (Under Review) Preprint DOI: 10.31219/osf.io/cnh5p
Anopheles-associated Beauveria nivea reduces mosquito infectivity to Plasmodium infection.
Vandana✻, Kona MP, Gupta S, Kumar J, Sankar H, Gupta Y, Singh S, Singh OP, Pandey KC
Journal of Biological Chemistry. (Under Review)